He was under treatment at Combined Military Hospital in Malir Cantonment.
He leaves behind a widow, three daughters and two sons.
Comedian, Umar Sharif said Akhtar had been like an elder brother to him and his advice had helped Sharif improve.
“I was planning to gather all the comedians in Pakistan to give a tribute to him, God had planned otherwise,” said Sharif.
Actress, Bushra Ansari said she always felt tremendous pride being compared to Akhtar, and the actor’s death has come as a great shock.
“All he did was work, to the point of illness” said Ansari.
Akhtar had recently been working on a new show Dolly ki Baraat with Ansari.
President Asif Ali Zardari, Prime Minister Yousaf Raza Gilani, Muttahida Qaumi Movement (MQM) chief Altaf Hussain and Sindh Governor Ishrtaul Ibad condoled his death.
Akhtar who was born in December 24 1950, was a skillful comedian, impersonator and host, and worked on hundreds of Pakistani TV dramas, films and stage shows.
Fluent in several languages including English, Moin Akhtar performed dozens of stage shows for urdu-speaking communities all over the world.
In recognition of his services in the field of performing art, he was awarded the President’s pride of performance and Tamgha-e-Imtiaz, one of Pakistan’s highest national awards.
Some of Akhtar’s famed TV series included:
Makaan no 47
Bandar road se Kemari